Note: All fields described in the sections below (after the properties file example) are required unless explicitly described as “optional.” In your own properties file, you would replace values surrounded with with the relevant information. Contact us on the CellProfiler forums if you need help with this. Note: CPA 2.0 is not compatible with properties files from CellProfiler Analyst version 1.0, but the two formats may be easily converted by hand. We suggest using Notepad on Windows, TextEdit on Mac OS, and Emacs on Linux. Note: When editing the properties file, it is important to use an editor that is capable of saving plain text. Settings that require a file path may be specified either as absolute or relative to the directory that the properties file is found in. Lines that begin with a # are ignored by CPA and may be used for comments.
Otherwise, you can create one manually, referring to the Properties_README or the example provided below as a template.Įach setting in the properties file is stored on a separate line in the form field = value(s), and the order of the settings is not important. If you use CellProfiler to produce the data to be analyzed in CPA, you can automatically generate a nearly complete properties file with, using the ExportToDatabase module. For applying the machine learning tool to identify a phenotype of interest, watch the tutorial on Classifying Cells with Machine Learning. properties file generated by CellProfiler c. Provides an example of selecting phenotypes using gating. It is selected and loaded upon startup of CPA. CellProfiler Analyst is a software tool that complements CellProfiler. This file can be stored anywhere on your computer. The properties file is a plain text file that contains the configuration information necessary for CPA to access your data and images.